While real-time reverse transcriptase–polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is the current standard for SARS-CoV-2 detection, saliva is an attractive alternative for diagnosis and screening due to ease of collection and minimal supply requirements.^1,2 Studies on the sensitivity of saliva-based SARS-CoV-2 molecular testing have shown considerable variability.³ We conducted a prospective, longitudinal study to investigate the testing timeframe that optimizes saliva sensitivity for SARS-CoV-2 detection.